Quantifying the fraction of glycine and alanine in β-sheet and helical conformations in spider dragline silk using solid-state NMR
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چکیده
منابع مشابه
Quantifying the fraction of alanine residues in an -helical conformation in hornet silk using solid-state NMR
Here, we demonstrate the first successful isotope labeling of Ala carbons in hornet silk produced by the larvae of Vespa (Vespinae, Vespidae) mandarinia. This labeled hornet silk was examined by high-resolution 13C solid-state NMR, and it was found that the fraction of Ala residues in a-helical conformations compared with Ala residues in the overall conformation of hornet silk can be quantitati...
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The molecular structure of spider dragline silk: folding and orientation of the protein backbone.
The design principles of spider dragline silk, nature's high-performance fiber, are still largely unknown, in particular for the noncrystalline glycine-rich domains, which form the bulk of the material. Here we apply two-dimensional solid-state NMR to determine the distribution of the backbone torsion angles (phi,psi) as well as the orientation of the polypeptide backbone toward the fiber at bo...
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A simple solid-state NMR method was used to study the structure of (13)C- and (15)N-enriched silk from two Australian orb-web spider species, Nephila edulis and Argiope keyserlingi. Carbon-13 and (15)N spectra from alanine- or glycine-labeled oriented dragline silks were acquired with the fiber axis aligned parallel or perpendicular to the magnetic field. The fraction of oriented component was ...
متن کاملDiffraction from the β-sheet crystallites in spider silk
We analyze the wide angle x-ray scattering from oriented spider silk fibers in terms of a quantitative scattering model, including both structural and statistical parameters of the β-sheet crystallites of spider silk in the amorphous matrix. The model is based on kinematic scattering theory and allows for rather general correlations of the positional and orientational degrees of freedom, includ...
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2008
ISSN: 1359-7345,1364-548X
DOI: 10.1039/b812928b